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In the 1930s, Frits Zernike, a Dutch physicist at the University of Groningen, created an optical design that could transform differences in phase to differences in amplitude. The development of phase contrast optics is a brilliant example of how basic research in theoretical optics led to a practical solution for viewing unstained transparent objects in the light microscope. The Zeiss optical works in Jena introduced phase contrast objectives and accessories in 1942, which transformed research in biology and medicine. For his invention and theory of image formation, Zernike won the Nobel prize in physics in 1953 (Fig. 7-4). Excellent descriptions of the technique are found in Bennett et al. (1951), Fran on (1961), Slayter (1976), Slayter and Slayter (1992), and Pluta (1989). In this chapter we first examine the process of image formation using the terminology and diagrams that are commonly employed to explain phase contrast optics. Then we examine the relative contributions of diffraction and differences in optical path length in generating the phase contrast image. vb.net pdf editor VB.NET PDF Library SDK to view, edit, convert, process PDF file for ...
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this may help you. Pdf Api for editing on Asp,net[^] PDF API for .NET [^]. It is worth noting that an effective cognitive-behavioural treatment that is derived from a cognitive-behavioural model does not prove that the model is correct since the treatment could be effective for a number of other, non-speci c reasons. Such data can only be taken as being consistent with the cognitive-behavioural model and providing indirect support for it. It is, however, possible to conclude that the maintenance model is incorrect if the therapy reverses the proposed maintaining mechanisms yet the disorder persists. Based on the early behavioural accounts, operant paradigms were implemented consisting of (a) isolating patients from reinforcers and (b) providing the reinforcers in response to speci ed criteria such as weight gain or calorie intake. At least 60 publications describing operant techniques for anorexia nervosa were published in the 1960s and 1970s and the literature has been thoroughly reviewed elsewhere (Halmi, 1985). Reviews indicate the effectiveness of operant conditioning in the short-term but the technique has questionable utility for long-term improvement (see Bemis, 1982). More recently, behaviour therapy plus education has been shown to be successful in a recent controlled study (Treasure et al., 1995), indicating the promise of this type of therapy. Cognitive-behavioural therapy, however, is considered to be indicated for AN in adults by 90% of clinicians (Herzog et al., 1992). As with the cognitive-behavioural models, the leading account of the cognitive-behavioural treatment of anorexia nervosa is that proposed by Garner and Bemis (1982, 1985). To date, the descriptions of this treatment have tended to be schematic rather than detailed, although two recent publications have provided rather more information (Pike, Loeb & Vitousek, 1996; Garner, Vitousek & Pike, 1997). vb.net pdf editor VS 2010 Editing a PDF File VB .NET -iTextSharp-VBForums
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